high-throughput molecular abundance data, predominantly gene expression data generated by dna microarray Search Results


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mirna microarray  (HTG Molecular)
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( A ) <t>qNPA</t> <t>microarray</t> was performed in triplicate on MCF10CA1a siCtrl cells and MCF10CA1a siβ4 cells at 72 hours post-transfection. The heat map depicts the 44 miRNAs undergoing a statistically significant change in expression following transient depletion of β4 subunit in this system. ( B ) qNPA microarray was performed in triplicate on two subclones of the MDA-MB-435/β4 transfectants (3A7 and 5B3), and two subclones of the MDA-MB-435/mock transfectants (6D2 and 6D7). The heat map depicts the 50 miRNAs undergoing a statistically significant change in expression following introduction of the β4 subunit into this system. ( C ) qNPA microarray was performed in triplicate on ten β4 positive and ten β4 negative invasive breast carcinomas. The heat map depicts the 74 miRNAs differentially expressed between tumor subsets. For all array analyses, a p-value < 0.05 and a ±1.2-fold change cut-off was applied. Color was assigned to each <t>miRNA</t> based on relative expression across samples.
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( A ) <t>qNPA</t> <t>microarray</t> was performed in triplicate on MCF10CA1a siCtrl cells and MCF10CA1a siβ4 cells at 72 hours post-transfection. The heat map depicts the 44 miRNAs undergoing a statistically significant change in expression following transient depletion of β4 subunit in this system. ( B ) qNPA microarray was performed in triplicate on two subclones of the MDA-MB-435/β4 transfectants (3A7 and 5B3), and two subclones of the MDA-MB-435/mock transfectants (6D2 and 6D7). The heat map depicts the 50 miRNAs undergoing a statistically significant change in expression following introduction of the β4 subunit into this system. ( C ) qNPA microarray was performed in triplicate on ten β4 positive and ten β4 negative invasive breast carcinomas. The heat map depicts the 74 miRNAs differentially expressed between tumor subsets. For all array analyses, a p-value < 0.05 and a ±1.2-fold change cut-off was applied. Color was assigned to each <t>miRNA</t> based on relative expression across samples.
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( A ) <t>qNPA</t> <t>microarray</t> was performed in triplicate on MCF10CA1a siCtrl cells and MCF10CA1a siβ4 cells at 72 hours post-transfection. The heat map depicts the 44 miRNAs undergoing a statistically significant change in expression following transient depletion of β4 subunit in this system. ( B ) qNPA microarray was performed in triplicate on two subclones of the MDA-MB-435/β4 transfectants (3A7 and 5B3), and two subclones of the MDA-MB-435/mock transfectants (6D2 and 6D7). The heat map depicts the 50 miRNAs undergoing a statistically significant change in expression following introduction of the β4 subunit into this system. ( C ) qNPA microarray was performed in triplicate on ten β4 positive and ten β4 negative invasive breast carcinomas. The heat map depicts the 74 miRNAs differentially expressed between tumor subsets. For all array analyses, a p-value < 0.05 and a ±1.2-fold change cut-off was applied. Color was assigned to each <t>miRNA</t> based on relative expression across samples.
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( A ) <t>qNPA</t> <t>microarray</t> was performed in triplicate on MCF10CA1a siCtrl cells and MCF10CA1a siβ4 cells at 72 hours post-transfection. The heat map depicts the 44 miRNAs undergoing a statistically significant change in expression following transient depletion of β4 subunit in this system. ( B ) qNPA microarray was performed in triplicate on two subclones of the MDA-MB-435/β4 transfectants (3A7 and 5B3), and two subclones of the MDA-MB-435/mock transfectants (6D2 and 6D7). The heat map depicts the 50 miRNAs undergoing a statistically significant change in expression following introduction of the β4 subunit into this system. ( C ) qNPA microarray was performed in triplicate on ten β4 positive and ten β4 negative invasive breast carcinomas. The heat map depicts the 74 miRNAs differentially expressed between tumor subsets. For all array analyses, a p-value < 0.05 and a ±1.2-fold change cut-off was applied. Color was assigned to each <t>miRNA</t> based on relative expression across samples.
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high-throughput sequencing  (Ribonomics Inc)
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( A ) <t>qNPA</t> <t>microarray</t> was performed in triplicate on MCF10CA1a siCtrl cells and MCF10CA1a siβ4 cells at 72 hours post-transfection. The heat map depicts the 44 miRNAs undergoing a statistically significant change in expression following transient depletion of β4 subunit in this system. ( B ) qNPA microarray was performed in triplicate on two subclones of the MDA-MB-435/β4 transfectants (3A7 and 5B3), and two subclones of the MDA-MB-435/mock transfectants (6D2 and 6D7). The heat map depicts the 50 miRNAs undergoing a statistically significant change in expression following introduction of the β4 subunit into this system. ( C ) qNPA microarray was performed in triplicate on ten β4 positive and ten β4 negative invasive breast carcinomas. The heat map depicts the 74 miRNAs differentially expressed between tumor subsets. For all array analyses, a p-value < 0.05 and a ±1.2-fold change cut-off was applied. Color was assigned to each <t>miRNA</t> based on relative expression across samples.
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Image Search Results


( A ) qNPA microarray was performed in triplicate on MCF10CA1a siCtrl cells and MCF10CA1a siβ4 cells at 72 hours post-transfection. The heat map depicts the 44 miRNAs undergoing a statistically significant change in expression following transient depletion of β4 subunit in this system. ( B ) qNPA microarray was performed in triplicate on two subclones of the MDA-MB-435/β4 transfectants (3A7 and 5B3), and two subclones of the MDA-MB-435/mock transfectants (6D2 and 6D7). The heat map depicts the 50 miRNAs undergoing a statistically significant change in expression following introduction of the β4 subunit into this system. ( C ) qNPA microarray was performed in triplicate on ten β4 positive and ten β4 negative invasive breast carcinomas. The heat map depicts the 74 miRNAs differentially expressed between tumor subsets. For all array analyses, a p-value < 0.05 and a ±1.2-fold change cut-off was applied. Color was assigned to each miRNA based on relative expression across samples.

Journal: Biology Open

Article Title: Effects of β4 integrin expression on microRNA patterns in breast cancer

doi: 10.1242/bio.20121628

Figure Lengend Snippet: ( A ) qNPA microarray was performed in triplicate on MCF10CA1a siCtrl cells and MCF10CA1a siβ4 cells at 72 hours post-transfection. The heat map depicts the 44 miRNAs undergoing a statistically significant change in expression following transient depletion of β4 subunit in this system. ( B ) qNPA microarray was performed in triplicate on two subclones of the MDA-MB-435/β4 transfectants (3A7 and 5B3), and two subclones of the MDA-MB-435/mock transfectants (6D2 and 6D7). The heat map depicts the 50 miRNAs undergoing a statistically significant change in expression following introduction of the β4 subunit into this system. ( C ) qNPA microarray was performed in triplicate on ten β4 positive and ten β4 negative invasive breast carcinomas. The heat map depicts the 74 miRNAs differentially expressed between tumor subsets. For all array analyses, a p-value < 0.05 and a ±1.2-fold change cut-off was applied. Color was assigned to each miRNA based on relative expression across samples.

Article Snippet: A novel qNPA based miRNA Microarray high throughput platform from High Throughput Genomics (HTG Molecular Diagnostics, Inc.; Tuscon, AZ, USA) was used to study 1050 mature miRNAs in human, rat, and mouse based upon the Sanger miRBase release 9.1.

Techniques: Microarray, Transfection, Expressing

( A ) Venn diagram of overlapping miRNAs that undergo differential expression in response to β4 across all three arrays. ( B ) Venn diagram of overlapping miRNA families that undergo differential expression in response to β4 across all three arrays.

Journal: Biology Open

Article Title: Effects of β4 integrin expression on microRNA patterns in breast cancer

doi: 10.1242/bio.20121628

Figure Lengend Snippet: ( A ) Venn diagram of overlapping miRNAs that undergo differential expression in response to β4 across all three arrays. ( B ) Venn diagram of overlapping miRNA families that undergo differential expression in response to β4 across all three arrays.

Article Snippet: A novel qNPA based miRNA Microarray high throughput platform from High Throughput Genomics (HTG Molecular Diagnostics, Inc.; Tuscon, AZ, USA) was used to study 1050 mature miRNAs in human, rat, and mouse based upon the Sanger miRBase release 9.1.

Techniques: Quantitative Proteomics

GeneChip derived mRNA levels were ranked from the most upregulated in β4 transfected cells to the most downregulated (x-axis, 1 to 12,300, respectively). Red shading indicates mRNA is upregulated in β4 transfectants, while blue shading indicates mRNA is downregulated. Each vertical black line represents a miRNA target. The left-to-right position of each black line indicates the relative position of the predicted target within the rank ordered mRNA list. ( A ) miR-92ab predicted target gene are enriched among mRNAs up-regulated in the β4 transfectants, as illustrated by the increasing number of black lines on the left side of each graphic and the positive running enrichment scores (ES) marked by the red lines (p = 0.028). No enrichment was detected for and miR-99ab/100. ( B ) miR-15abc/16/16abc/195/322/424/497/1907 (p = 0.039), miR-23abc/23b-3p (p = 0/034), miR-27abc/27a-3p (p = 0.003), and miR-30abcdef/30abe-5p/384-5p (p = 0.0) predicted target genes are enriched among mRNAs up-regulated in the β4 transfectants.

Journal: Biology Open

Article Title: Effects of β4 integrin expression on microRNA patterns in breast cancer

doi: 10.1242/bio.20121628

Figure Lengend Snippet: GeneChip derived mRNA levels were ranked from the most upregulated in β4 transfected cells to the most downregulated (x-axis, 1 to 12,300, respectively). Red shading indicates mRNA is upregulated in β4 transfectants, while blue shading indicates mRNA is downregulated. Each vertical black line represents a miRNA target. The left-to-right position of each black line indicates the relative position of the predicted target within the rank ordered mRNA list. ( A ) miR-92ab predicted target gene are enriched among mRNAs up-regulated in the β4 transfectants, as illustrated by the increasing number of black lines on the left side of each graphic and the positive running enrichment scores (ES) marked by the red lines (p = 0.028). No enrichment was detected for and miR-99ab/100. ( B ) miR-15abc/16/16abc/195/322/424/497/1907 (p = 0.039), miR-23abc/23b-3p (p = 0/034), miR-27abc/27a-3p (p = 0.003), and miR-30abcdef/30abe-5p/384-5p (p = 0.0) predicted target genes are enriched among mRNAs up-regulated in the β4 transfectants.

Article Snippet: A novel qNPA based miRNA Microarray high throughput platform from High Throughput Genomics (HTG Molecular Diagnostics, Inc.; Tuscon, AZ, USA) was used to study 1050 mature miRNAs in human, rat, and mouse based upon the Sanger miRBase release 9.1.

Techniques: Derivative Assay, Transfection